Understanding Ge Gen Qin Lian Tang
'Ge-Gen-Qin-Lian' Decoction derived from 'Shang-Han-Lun'
compiled by Zhang Zhongjing. It is widely used in the treatment of acute
gastroenteritis, bacillary dysentery, virus diarrhea. This paper describes a
sensitive and specific assay for the determination of the 11-marker compounds
using ultra performance liquid chromatography (UPLC). To develop an UPLC method
for simultaneous determination of 11 bioactive compounds in 'Ge-Gen-Qin-Lian'
preparations. The chromatography analysis was performed on an Agilent Proshell
120 EC-C18 column (4.6 × 50 mm, 2.7 μm) at 30°C with a gradient
elution of methanol, 0.5% formic acid and 0.5% ammonium acetate at a flow rate
1.0 ml/min and UV detected at 270 nm. All calibration curves showed good linear
regression (r ≥ 0.9993) within tested ranges. Limits of detection (LOD)
and limits of quantification (LOQ) fell in the range between 0.0691-1.04 μg/ml
and 0.23-3.43 μg/ml, respectively. The mean recovery of each herbal medicine
ranged from 96.60 to 102.11%. The method was validated for repeatability,
precision, stability, accuracy, and selectivity. The validated method was
successfully applied to simultaneous analysis of these active components in
'Ge-Gen-Qin-Lian' decoction.
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